Cytocell
offers a complete range of Satellite probes available in the
Aquariusliquid format. The probes are chromosome specific sequences
generated from highly repeated human satellite DNA sequences located in
the centromeric, pericentromeric or heterochromatic regions of each
chromosome. They are available independently and directly labelled in
either a red or green fluorophore (Texas Red or FITC spectra
respectively).
The probes are produced in a concentrated form to
allow the mixing, if required, of up to 3 other satellite probes in the
same hybridisation.
Detailed Protocol
The kits are supplied in an economical 5 test format and come complete
with hybridisation solution and DAPI counterstain. The protocol is
rapid and simple and follows a co-denaturation of the FISH probe and
target DNA simultaneously.
The probes are designed for use in fluorescence in situ hybridisation
analysis of human metaphase chromosomes from cultured peripheral blood
cells.