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 AneuCyte® FAST FISH and AneuCyte® Prenatal Kits :
Prenatal Fluorescence in Situ Hybridisation (FISH) assay for the
rapid and accurate detection of the most common foetal chromosome
disorders.
The kits contain fluorescent probes for easy
identification of trisomy 21, 18 and 13 present in Down, Edward and
Patau syndromes as well as sex chromosome aneuploidies (Klinefelter and Turner syndromes).
The probe mixtures are designed for FISH on uncultured amniotic fluid samples.
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two probe sets for two separate hybridisations (3 formats available : 10, 30 or 50 tests)Probe Set 1 :DXZ1 (X centromere a-satellite) labelled with a Green fluorophore (FITC spectrum) DYZ3 (Y centromere a-satellite) labelled with an Orange fluorophore (TRITC/Orange spectrum) D18Z1 (18 centromere a-satellite) labelled with a Blue fluorophore (Aqua spectrum) Probe Set 2 :13q14.3 unique sequence (including the markers D13S915, D13S1155, D13S1195) labelled with a Green fluorophore (FITC spectrum) 21q22.2
unique sequence (including the markers D21S270, D21S1867, D21S337,
D21S1425, D21S1444, D21S341) labelled with an Orange fluorophore
(TRITC/Orange spectrum) DAPI/Antifade ES: 0.125 mg/ml DAPI (4,6-diamidino-2-phenylindole) in vectashield, 500 ml per vial
AneuCyte® Complete kit for X, Y, 18, 13 and 21 - Separated kits :
2h hybridisation only !
Reference
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Fluorochrome*
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Probe sets**
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Format
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 LPF001
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Green/Orange/Blue
and
Green/Orange
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X, Y, 18, 13 and 21 :
DXZ1/DYZ3/D18Z1
and
13q14.2/21q22.13
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10 tests
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LPF001-30
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Green/Orange/Blue
and
Green/Orange
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X, Y, 18, 13 and 21 :
DXZ1/DYZ3/D18Z1
and
13q14.2/21q22.13
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30 tests
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LPF001-50
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Green/Orange/Blue
and
Green/Orange
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X, Y, 18, 13 and 21 :
DXZ1/DYZ3/D18Z1
and
13q14.2/21q22.13
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50 tests
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LPF002
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Green/Orange/Blue
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centromeres X, Y et 18 :
DXZ1/DYZ3/D18Z1
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10 tests
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LPF003
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Green/Orange
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Sequences uniques
13 et 21 : 13q14.2/21q22.13
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10 tests
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AneuCyte® Complete kit for X, Y, 18, 13 and 21 - Separated kits :
overnight hybridisation protocol |
| Reference | Fluorochrome* | Probe sets** | Format
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| LPA001 | Green/Orange/Blue
and
Green/Orange | X, Y, 18, 13 and 21 :
DXZ1/DYZ3/D18Z1
and
13q14.2/21q22.13 | 10 tests
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LPA001-30
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Green/Orange/Blue
and
Green/Orange
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X, Y, 18, 13 and 21 :
DXZ1/DYZ3/D18Z1
and
13q14.2/21q22.13
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30 tests
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LPA001-50
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Green/Orange/Blue
and
Green/Orange
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X, Y, 18, 13 and 21 :
DXZ1/DYZ3/D18Z1
and
13q14.2/21q22.13
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50 tests
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| LPA005 | Blue/Green/Orange | 18, 13 et 21 :
D18Z1/13q14.2/21q22.13 | 10 tests
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| LPA002 | Green/Orange/Blue | centromeres X, Y and 18 :
DXZ1/DYZ3/D18Z1 | 10 tests
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| LPA003 | Green/Orange | Unique sequence
13 and 21 :
13q14.2/21q22.13 | 10 tests
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| LPA004 | Blue | 18 centromere : D18Z1 | 10 tests
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* Green : triple filtre DAPI/FITC/TRITC ; Orange : triple filtre DAPI/FITC/TRITC ; Blue : filtre Aqua (spectre Aqua or DEAC)
** 13q14.2 including markers D13S915, D13S1155, RB1 and D13S1195
** 21q22.13 including markers D21S270, D21S1867, D21S337, D21S1425, D21S1444 and D21S341
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 X,Y,18 (46,XY)
 X,18 (46,XX) 13 et 21 (normal)
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